GTM Research Reserve is located south of the City of Jacksonville (Duval County) in St. Johns County and Flagler County on the northeast coast of Florida. GTM Research Reserve is geographically separated into a northern and southern component, separated by the City of St. Augustine. The northern component (referred to locally as Guana) is associated with the Tolomato and Guana River estuaries and the southern component is associated with the Matanzas River.

The northern component consists of:

• Guana River Marsh Aquatic Preserve.
• Guana River Wildlife Management Area.
• Stokes Landing Conservation Area.
• Deep Creek State Forest.

The GTM Environmental Education Center (EEC) is located in the northern component of GTM Research Reserve ten miles north of St. Augustine on State Road A1A in Ponte Vedra Beach, and serves as the administrative, education, research and stewardship facilities for the northern component of GTM Research Reserve.

The southern component of GTM Research Reserve consists of:

• Pellicer Creek Aquatic Preserve.
• Faver-Dykes State Park.
• Washington Oaks Gardens State Park.
• Moses Creek Conservation Area.
• Pellicer Creek Conservation Area.
• Fort Matanzas National Monument.
• Princess Place Preserve.
• The River to Sea Preserve at Marineland.
• Other state sovereign submerged lands adjacent to the Matanzas River within its boundary.

There is a smaller office building on AIA within the River to the Sea Preserve in Marineland. The Board of Trustees of the Internal Improvement Trust Fund (the Trustees) has designated CAMA to manage Guana River Marsh Aquatic Preserve (excluding Guana River Wildlife Management Area) and Pellicer Creek Aquatic Preserve.